Study of thiol-disulfide exchange in human erythrocytes under hypoxia conditions

Abstract

The content of reduced glutathione and SH-groups of erythrocyte proteins under conditions of oxidative and metabolic stress and hypoxia was experimentally investigated. It is shown that the content of these indicators significantly depends on the presence and amount of glucose in the incubation medium. A mathematical metabolic model was created that reproduces the work of the system of thiol-disulfide exchange in erythrocytes and deglutationation. The model was created in the COPASI program and consists of 70 differential equations. The state of hypoxia was simulated by changing the value of the partial pressure of oxygen. It is shown that the simulation results reproduce the experimental data obtained in the absence of oxidative and metabolic stress.